Application of next-generation sequencing to detect variants of drug-resistant Mycobacterium tuberculosis: genotype–phenotype correlation

Table 3 Agreement between phenotypic and genotypic test results

Genotypic result	Phenotypic result (N)		Cohen’s Kappa (95% confidence interval)
Genotypic result	Resistant	Susceptible	Cohen’s Kappa (95% confidence interval)
INH
Mutated (N = 12)	12	0	0.824 (0.632–1.000)
Unmutated (N = 24)	3	21	0.824 (0.632–1.000)
RFP
Mutated (N = 11)	11	0	1.000 (1.000–1.000)
Unmutated (N = 25)	0	25	1.000 (1.000–1.000)
EMB
Mutated (N = 8)	7	1	0.916 (0.753–1.000)
Unmutated (N = 28)	0	28
PZA
Mutated (N = 4)	4	0	0.609 (0.247–0.970)
Unmutated (N = 4)	4	28
SM
Mutated (N = 4)	4	0	0.491 (0.118–0.683)
Unmutated (N = 32)	6	26
AMK, KM
Mutated (N = 0)	0	0	N/A
Unmutated (N = 36)	2	34
FQ
Mutated (N = 4)	4	0	0.769 (0.458–1.000)
Unmutated (N = 32)	2	30

INH isoniazid, RFP rifampin, EMB ethambutol, PZA pyrazinamide, SM streptomycin, AMK amikacin, KM kanamycin, FQ fluoroquinolones

ISSN: 1476-0711