Fig. 6
Establishment of a qPCR assay as a comparative method for GBS detection. A The performance of qPCR detection method was validated by DNA concentrations in the range of 1 to 105 copies μL−1. B A standard curve of qPCR assay for quantification of GBS. CT values were plotted against copy numbers of GBS plasmid DNA. Data represents mean ± SD from triplicate measurements. ∆Rn = Rnf-Rnb, where Rnf was the fluorescence emission of the product at each time point and Rnb was the fluorescence emission of the baseline