Fig. 3

Relative aptamer binding force against the low degree polymeric (1 → 3)-β-D-glucans. A The enzyme-linked oligonucleotide assay (ELONA) was used to analyze the relative binding force of the different single-stranded DNA (ssDNA) pools of the (1 → 3)-β-D-glucans. L stands for initial ssDNA library. B The 40 random-selected E. coli DH5α strains containing pUC19-aptamers were amplified to yield double-stranded DNA aptamers by PCR and identified by agarose gel. C ELONA was used to analyze the relative binding strength of the 40 randomly picked single aptamers from the 8th round of collection to the (1 → 3)-β-D-glucans. All data are shown as the means ± standard deviation, and experiments were performed in triplicates. The aptamers with topmost relative affinity are indicated by arrows ( “A1” ~ “A6” from left to right)