Table 1 Primer combinations used for detection of ESBL-encoding genes
Target Gene | Primer Name | Primer Sequence | Annealing Temp(°C) | Band Size (bp) | Source |
|---|---|---|---|---|---|
TEM-type | TEM-F | 5′-atgagtattcaacatttc cg-3′ | 50 | 867 | [23] |
TEM-R | 5′-ccaatgcttaatcagtga cg-3′ | ||||
SHV-type | SHV-F | 5′-ttcgcctgtgtattatctccctg-3′ | 50 | 854 | [24] |
SHV-R | 5′-ttagcgttgccagtgytcg-3′ | ||||
OXA-1 | OXA-F | 5'-atgaaaaacacaatacatatcaacttc gc-3′ | 62 | 280 | [23] |
OXA-R | 5′-gtgtgtttagaatggtgatcgcat t-3′ | ||||
CTX-M-type | CTX-M-F | 5′-atgtgcagyaacagtaarrgtkatg gc-3′ | 60 | 593 | [23] |
CTX-M-R | 5′-tgggtraartargtsaacagaaycagc gg-3′ |