Table 1 Oligonucleotide primers used in cDNA synthesis and amplification by qPCR
Gene | Primer (5’→3’) | Amplicon size (bp) | Tm (°C) | Reference |
|---|---|---|---|---|
icaA (for S. epidermidis) | F: TGCACTCAATGAGGGAATCA R: TAACTGCGCCTAATTTTGGATT | 134 | 56 | [44] |
16Â S rRNA (Reference gene) | F: GGGCTACACACGTGCTACAA R: GTACAAGACCCGGGAACGTA | 176 | 56 | [44] |
icaA (for S. aureus ATCC 43,300) | F: ACACTTGCTGGCGCAGTCAA R: TCTGGAACCAACATCCAACA | 188 | 56 | [72] |