Genomic diversity of β-lactamase producing Pseudomonas aeruginosa in Iran; the impact of global high-risk clones

Ahmadi, Nazila; Salimizand, Himen; Zomorodi, Abolfazl Rafati; Abbas, Jalileh Ebn; Ramazanzadeh, Rashid; Haghi, Fakhri; Hassanzadeh, Sepideh; Jahantigh, Mojdeh; Shahin, Mojtaba

doi:10.1186/s12941-024-00668-5

Annals of Clinical Microbiology and Antimicrobials

Table 1 Primers used in this study

From: Genomic diversity of β-lactamase producing Pseudomonas aeruginosa in Iran; the impact of global high-risk clones

Genes	Primer sequence	Cycling Conditions	Product length (bp)	Reference
blaOXA-48	GCGTGGTTAAGGATGAACAC CATCAAGTTCAACCCAACCG	94 °C-10΄, 36 cycles of 94 °C-30˝, 52 °C-40˝, 72 °C-50˝, with 72 °C-5΄	438	[17]
blaIMP	GAAGGYGTTTATGTTCATAC GTAMGTTTCAAGAGTGATGC		232	[17]
blaVIM	GTTTGGTCGCATATCGCAAC AATGCGCAGCACCAGGATAG		390	[17]
blaNDM	GGTTTGGCGATCTGGTTTTC CGGAATGGCTCATCACGATC		621	[17]
blaGES	AGTCGGCTAGACCGGAAA GTTTGTCCGTGCTCAGGAT		399	[17]
blaSPM	AAAATCTGGGTACGCAAACG ACATTATCCGCTGGAACAGG		271	[17]
blaDIM	GCTTGTCTTCGCTTGCTAACG CGTTCGGCTGGATTGATTTG		699	[17]
blaBIC	TATGCAGCTCCTTTAAGGGC TCATTGGCGGTGCCGTACAC		537	[17]
blaSIM	TACAAGGGATTCGGCATCG TAATGGCCTGTTCCCATGTG		570	[17]
blaGIM	TCGACACACCTTGGTCTGAA AACTTCCAACTTTGCCATGC		477	[17]
blaAIM	CTGAAGGTGTACGGAAACAC GTTCGGCCACCTCGAATTG		322	[17]
TEM	CATTTCCGTGTCGCCCTTATTC CGTTCATCCATAGTTGCCTGAC	94 °C-10΄, 30 cycles of 94 °C-40˝, 60 °C-40˝, 72 °C-60˝, with 72 °C-7΄	800	[14]
SHV	AGCCGCTTGAGCAAATTAAAC ATCCCGCAGATAAATCACCAC		713	[14]
OXA-1	GGCACCAGATTCAACTTTCAAG GACCCCAAGTTTCCTGTAAGTG		564	[14]
CTX-M group 1	TTAGGAARTGTGCCGCTGYA CGATATCGTTGGTGGTRCCAT		688	[14]
CTX-M group 2	CGTTAACGGCACGATGAC CGATATCGTTGGTGGTRCCAT		404	[14]
CTX-M group 8	AACRCRCAGACGCTCTAC TCGAGCCGGAASGTGTYAT		326	[14]
CTX-M group 9	TCAAGCCTGCCGATCTGGT TGATTCTCGCCGCTGAAG		561	[14]
ACC	AACAGCCTCAGCAGCCGGTTA TTCGCCGCAATCATCCCTAGC	94 °C-3΄, 25 cycles of 94 °C-30˝, 64 °C-30˝, 72 °C-60˝, with 72 °C-7΄	346	[16]
ACT1-MIR1	TCGGTAAAGCCGATGTTG CGG CTTCCACTGCGGCTGCCAGTT		302	[16]
FOX	AACATGGGGTATCAGGGAGATG CAAAGCGCGTAACCGGATTGG		190	[16]
MOX	GCTGCTCAAGGAGCACAGGAT CACATTGACATAGGT GTGGTGC		520	[16]
LAT	TGGCCAGAACTGACAGGCAAA TTTCTCCTGAACGTGGCTGGC		462	[16]
DHA	AACTTTCACAGGTGTGCTGGGT CCGTACGCATACTGGCTTTGC		405	[16]
acsA*	GCCACACCTACATCGTCTAT GTGGACAACCTCGGCAACCT	94 °C-3΄, 35 cycles of 96 °C-60˝, 55 °C-60˝, 72 °C-60˝, with 72 °C-7΄	390	[20]
aroE*	ATGTCACCGTGCCGTTCAAG TGAAGGCAGTCGGTTCCTTG		495	[20]
guaA*	AGGTCGGTTCCTCCAAGGTC TCAAGTCGCACCACAACGTC		372	[20]
mutL*	AGAAGACCGAGTTCGACCAT ATGACTTCCTCTATGGCACC		441	[20]
nuoD*	ACGGCGAGAACGAGGACTAC TTCACCTTCACCGACCGCCA		366	[20]
ppsA*	GGTGACGACGGCAAGCTGTA TCCTGTGCCGAAGGCGATAC		369	[20]
trpE*	TTCAACTTCGGCGACTTCCA GGTGTCCATGTTGCCGTTCC		441	[20]

^{*Primers used for multi−locus sequence typing (MLST)}

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ISSN: 1476-0711

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