Dissemination of mcr-1 and β-lactamase genes among Pseudomonas aeruginosa: molecular characterization of MDR strains in broiler chicks and dead-in-shell chicks infections

Table 1 Oligonucleotides used in this study

Target gene	Primer direction and sequence	Amplicon size (bp)	References
16S rRNA	F: GGGGGATCTTCGGACCTCA R: TCCTTAGAGTGCCCACCCG	956	[31]
lasB	F: GGAATGAACGAAGCGTTCTCCGAC R: TTGGCGTCGACGAACACCTCG	284	[33]
toxA	F: CTGCGCGGGTCTATGTGCC R: GATGCTGGACGGGTCGAG	270	[33]
exoS	F: CGTCGTGTTCAAGCAGATGGTGCTG R: CCGAACCGCTTCACCAGGC	444	[33]
int1	F: GCCTTGCTGTTCTTCTACGG R: GATGCCTGCTTGTTCTACGG	565	[34]
bla_TEM	F: ATCAGCAATAAACCAGC R: CCCCGAAGAACGTTTTC	516	[35]
bla_SHV	F: AGGATTGACTGCCTTTTTG R: ATTTGCTGATTTCGCTCG	392	[35]
bla_CTX-M	F: ATG TGC AGY ACC AGT AAR GTK ATG GC R: TGG GTR AAR TAR GTS ACC AGA AYC AGC GG	593	[36]
bla_OXA-10	F:TATCGCGTGTCTTTCGAGTA R: TTAGCCACCAATGATGCCC	760	[37]
bla_VEB-1	F: CGACTTCCATTTCCCGATGC R: GGACTCTGCAACAAATACGC	642	[37]
bla_OXA-2	F: GCCAAAGGCACGATAGTTGT R: GCGTCCGAGTTGACTGCCGG	700	[38]
mcr-1	F: CGGTCAGTCCGTTTGTTC R:CTTGGTCGGTCTGTA GGG	309	[39]

ISSN: 1476-0711