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Table 5 Results of biofilm formation abilities by MTP method using the ELISA plate reader at 560 nm

From: Dissemination of mcr-1 and β-lactamase genes among Pseudomonas aeruginosa: molecular characterization of MDR strains in broiler chicks and dead-in-shell chicks infections

Cut-off value calculation of MTP assay

OD results for 55 samples using the ELISA plate reader at 560 nm (n = 55)

Biofilm formation ability

ODC > 0.4

(4 × ODc < ODi)

1 = 1.326

2 = 1.043

3 = 0.814

4 = 0.750

5 = 0.440

5 (9.1%) strongly adherent isolates

0.2 < OD < 0.4

(2 × ODc < ODi ≤ 4 × ODc),

0.374

1 (1.82%) moderately adherent isolates

0.1 < ODNC < 0.2

(ODc < ODi ≤ 2 × ODc),

1 = 0.134

2 = 0.114

2 (3.6%) weakly adherent isolates

 < 0.1

(ODi < ODc)

0.064 and 0.001

47 (85.5%) nonadherent isolates

  1. MPA Microtiter Plate Assay, ODc optical density of control, ODi optical density of the samples, ODNC optical density of negative control
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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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