Table 1 Primers sequences and their futures for detection of β-lactamase encoding genes
Genes | Primers (5’→3’) | Amplicon size (bp) | Tm (°C) | References |
|---|---|---|---|---|
blaTEM | F: AGTATTCAACATTTYCGTGT R: TAATCAGTGAGGCACCTATCTC | 847 | 56 | [5] |
blaSHV | F: ATGCGTTATATTCGCCTGTG R: TTAGCGTTGCCAGTGCTC | 843 | 55 | |
blaCTX−M1 | F: GCGTGATACCACTTCACCTC R: TGAAGTAAGTGACCAGAATC | 260 | ||
blaCTX−M2 | F: TGATACCACCACGCCGCTC R: TATTGCATCAGAAACCGTGGG | 341 | ||
blaGES | F: ATGCGCTTCATTCACGCAC R: CTATTTGTCCGTGCTCAGGA | 863 | 56 | [4] |
blaVEB | F: ATTTCCCGATGCAAAGCGT R: TTATTCCGGAAGTCCCTGT | 542 | 55 | |
blaPER−2 | F: ATGAATGTCATCACAAAATG R: TCAATCCGGACTCACT | 927 | 50 | |
blaIMP | F: CATGGTTTGGTGGTTCTTGT R: ATAATTTGGCGGACTTTGGC | 488 | 56 | |
blaVIM | F: ATTGGTCTATTTGACCGCGTC R: TGCTACTCAACGACTGAGCG | 780 | 58 | |
blaNDM−1 | F: GAGATTGCCGAGCGACTTG R: CGAATGTCTGGCAGCACACTT | 497 | 54 | |
blaOXA−23 | F: GATCGGATTGGAGAACCAGA R: ATTTCTGACCGCATTTCCAT | 501 | 52 | |
blaOXA−24 | F: GGTTAGTTGGCCCCCTTAAA R: AGTTGAGCGAAAAGGGGATT | 246 | ||
blaOXA−51 | F: TAATGCTTTGATCGGCCTTG R: TGGATTGCACTTCATCTTGG | 353 | ||
blaOXA−58 | F: AAGTAT TGGGGCTTGTGCTG R: CCCCTCTGCGCTCTACATAC | 599 | ||
blaKPC | F: CGTTCTTGTCTCTCATGGCC R: CCTCGCTGTGCTTGTCATCC | 796 | 52 | [6] |
blaOXA−48 | F: TTGGTGGCATCGATTATCGG R: GAGCACTTCTTTTGTGATGGC | 743 | 57 |