Table 2 List of primers used in plasmid typing studies [7]
Replicon Type | Primers (5’\(\rightarrow\)3’) | Target Region | EMBL Accesion Number | Amplicon size (bp) |
|---|---|---|---|---|
HI1 | F: GGAGCGATGGATTACTTCAGTAC | parA-parB | AF250878 | 471 |
R: TGCCGTTTCACCTCGTGAGTA | ||||
HI2 | F: TTTCTCCTGAGTCACCTGTTAACAC | Iterons | BX664015 | 644 |
R: GGCTCACTACCGTTGTCATCCT | ||||
I1 | F: CGAAAGCCGGACGGCAGAA | RNAI | M20413 | 139 |
R: TCGTCGTTCCGCCAAGTTCGT | ||||
X | F: AACCTTAGAGGCTATTTAAGTTGCTGAT | orig | Y00768 | 376 |
R: TGAGAGTCAATTTTTATCTCATGTTTTAGC | ||||
L/M | F: GGATGAAAACTATCAGCATCTGAAG | repA, B, C | U27345 | 785 |
R: CTGCAGGGGCGATTCTTTAGG | ||||
N | F: GTCTAACGAGCTTACCGAAG | repA | NC_003292 | 559 |
R: GTTTCAACTCTGCCAAGTTC | ||||
FIA | F: CCATGCTGGTTCTAGAGAAGGTG | Iterons | J01724 | 462 |
R: GTATATCCTTACTGGCTTCCGCAG | ||||
FIB | F: GGAGTTCTGACACACGATTTTCTG | repA | M26308 | 702 |
R: CTCCCGTCGCTTCAGGGCATT | ||||
W | F: CCTAAGAACAACAAAGCCCCCG | repA | U12441 | 242 |
R: GGTGCGCGGCATAGAACCGT | ||||
Y | F: AATTCAAACAACACTGTGCAGCCTG | repA | K02380 | 765 |
R: GCGAGAATGGACGATTACAAAACTTT | ||||
P | F: CTATGGCCCTGCAAACGCGCCAGAAA | Iterons | M20134 | 534 |
R: TCACGCGCCAGGGCGCAGCC | ||||
FIC | F: GTGAACTGGCAGATGAGGAAGG | repA2 | AH003523 | 262 |
R: TTCTCCTCGTCGCCAAACTAGAT | ||||
A/C | F: GAGAACCAAAGACAAAGACCTGGA | repA | X73674 | 465 |
R: ACGACAAACCTGAATTGCCTCCTT | ||||
T | F: TTGGCCTGTTTGTGCCTAAACCAT | repA | K00053 | 750 |
R: CGTTGATTACACTTAGCTTTGGAC | ||||
FIIs | F: CTGTCGTAAGCTGATGGC | repA | AE006471 | 270 |
R: CTCTGCCACAAACTTCAGC | ||||
FrepB | F: TGATCGTTTAAGGAATTTTG | RNAI/repA | AY234375 | 270 |
R: GAAGATCAGTCACACCATCC | ||||
K/B | F: GCGGTCCGGAAAGCCAGAAAAC | RNAI | M93063 | 160 |
R: TCTTTCACGAGCCCGCCAAA | ||||
B/O | R: TCTGCGTTCCGCCAAGTTCGA | RNAI | M28718 | 159 |