Fig. 1

Procedure of nanopore targeted sequencing. The clinical application of the nanopore targeted sequencing (NTS) workflow, which consists of five parts. (1) Sample collection: cerebrospinal fluid of suspected meningitis patients was collected. (2) Nucleic acid extraction: cerebrospinal fluid sample DNA and RNA nucleic acid extraction. (3) Targeted pathogen enrichment: specific polymerase chain reaction (PCR) targeted enrichment of target pathogenic microorganisms. (4) Amplicon library preparation and mixed library: libraries were constructed for all samples using an SQK-PBK004 kit and library mixing was performed. (5) Sequencing and data analysis: library pools were loaded onto Gridion×5 sequencer for 1 h, and original data was analyzed